{
 "cells": [
  {
   "cell_type": "markdown",
   "id": "2f04eee0-5928-4e74-a754-6dc2e528810c",
   "metadata": {},
   "source": [
    "# McCartneyBodyFat"
   ]
  },
  {
   "cell_type": "markdown",
   "id": "a3f514a3-772c-4a14-afdf-5a8376851ff4",
   "metadata": {},
   "source": [
    "## Index\n",
    "1. [Instantiate model class](#Instantiate-model-class)\n",
    "2. [Define clock metadata](#Define-clock-metadata)\n",
    "3. [Download clock dependencies](#Download-clock-dependencies)\n",
    "5. [Load features](#Load-features)\n",
    "6. [Load weights into base model](#Load-weights-into-base-model)\n",
    "7. [Load reference values](#Load-reference-values)\n",
    "8. [Load preprocess and postprocess objects](#Load-preprocess-and-postprocess-objects)\n",
    "10. [Check all clock parameters](#Check-all-clock-parameters)\n",
    "10. [Basic test](#Basic-test)\n",
    "11. [Save torch model](#Save-torch-model)\n",
    "12. [Clear directory](#Clear-directory)\n"
   ]
  },
  {
   "cell_type": "markdown",
   "id": "d95fafdc-643a-40ea-a689-200bd132e90c",
   "metadata": {},
   "source": [
    "Let's first import some packages:"
   ]
  },
  {
   "cell_type": "code",
   "execution_count": 5,
   "id": "4adfb4de-cd79-4913-a1af-9e23e9e236c9",
   "metadata": {
    "execution": {
     "iopub.execute_input": "2025-04-07T17:47:34.133350Z",
     "iopub.status.busy": "2025-04-07T17:47:34.132902Z",
     "iopub.status.idle": "2025-04-07T17:47:35.476506Z",
     "shell.execute_reply": "2025-04-07T17:47:35.476150Z"
    }
   },
   "outputs": [],
   "source": [
    "import os\n",
    "import inspect\n",
    "import shutil\n",
    "import json\n",
    "import torch\n",
    "import pandas as pd\n",
    "import pyaging as pya"
   ]
  },
  {
   "cell_type": "markdown",
   "id": "145082e5-ced4-47ae-88c0-cb69773e3c5a",
   "metadata": {},
   "source": [
    "## Instantiate model class"
   ]
  },
  {
   "cell_type": "code",
   "execution_count": 6,
   "id": "8aa77372-7ed3-4da7-abc9-d30372106139",
   "metadata": {
    "execution": {
     "iopub.execute_input": "2025-04-07T17:47:35.478214Z",
     "iopub.status.busy": "2025-04-07T17:47:35.477997Z",
     "iopub.status.idle": "2025-04-07T17:47:35.484695Z",
     "shell.execute_reply": "2025-04-07T17:47:35.484415Z"
    }
   },
   "outputs": [
    {
     "name": "stdout",
     "output_type": "stream",
     "text": [
      "class McCartneyBodyFat(pyagingModel):\n",
      "    def __init__(self):\n",
      "        super().__init__()\n",
      "\n",
      "    def preprocess(self, x):\n",
      "        return x\n",
      "\n",
      "    def postprocess(self, x):\n",
      "        return torch.sigmoid(x)\n",
      "\n"
     ]
    }
   ],
   "source": [
    "def print_entire_class(cls):\n",
    "    source = inspect.getsource(cls)\n",
    "    print(source)\n",
    "\n",
    "print_entire_class(pya.models.McCartneyBodyFat)\n"
   ]
  },
  {
   "cell_type": "code",
   "execution_count": 7,
   "id": "78536494-f1d9-44de-8583-c89a310d2307",
   "metadata": {
    "execution": {
     "iopub.execute_input": "2025-04-07T17:47:35.485963Z",
     "iopub.status.busy": "2025-04-07T17:47:35.485866Z",
     "iopub.status.idle": "2025-04-07T17:47:35.487577Z",
     "shell.execute_reply": "2025-04-07T17:47:35.487295Z"
    }
   },
   "outputs": [],
   "source": [
    "model = pya.models.McCartneyBodyFat()"
   ]
  },
  {
   "cell_type": "markdown",
   "id": "51f8615e-01fa-4aa5-b196-3ee2b35d261c",
   "metadata": {},
   "source": [
    "## Define clock metadata"
   ]
  },
  {
   "cell_type": "code",
   "execution_count": 8,
   "id": "6601da9e-8adc-44ee-9308-75e3cd31b816",
   "metadata": {
    "execution": {
     "iopub.execute_input": "2025-04-07T17:47:35.488812Z",
     "iopub.status.busy": "2025-04-07T17:47:35.488727Z",
     "iopub.status.idle": "2025-04-07T17:47:35.490718Z",
     "shell.execute_reply": "2025-04-07T17:47:35.490459Z"
    }
   },
   "outputs": [],
   "source": [
    "model.metadata[\"clock_name\"] = \"mccartneybodyfat\"\n",
    "model.metadata[\"data_type\"] = \"DNA methylation\"  # Paper: The predictors use DNA methylation states at CpG sites.\n",
    "model.metadata[\"species\"] = \"Homo sapiens\"  # Paper: Generation Scotland is a population-based cohort of human participants.\n",
    "model.metadata[\"year\"] = 2018\n",
    "model.metadata[\"approved_by_author\"] = \"⌛\"\n",
    "model.metadata[\"citation\"] = \"McCartney, D. L., et al. “Epigenetic prediction of complex traits and death.” Genome Biology 19, 136 (2018).\"\n",
    "model.metadata[\"doi\"] = \"https://doi.org/10.1186/s13059-018-1514-1\"\n",
    "model.metadata[\"notes\"] = \"Whole-blood DNAm LASSO score for body fat percentage, trained in Generation Scotland on an age-, sex-, and ancestry-adjusted phenotype residual and evaluated out of sample in LBC1936.\"\n",
    "model.metadata[\"research_only\"] = None\n",
    "model.metadata[\"tissue\"] = [\"whole blood\"]  # Paper: Stored DNA from baseline blood samples was used to build the predictors.\n",
    "model.metadata[\"predicts\"] = [\"body fat percentage\"]  # Paper: The study developed a DNAm predictor for body fat percentage.\n",
    "model.metadata[\"training_target\"] = [\"body fat percentage\"]  # Paper: The body fat percentage phenotype was regressed on age, sex and ten genetic principal components; its residual was the LASSO outcome.\n",
    "model.metadata[\"unit\"] = [\"unitless\"]  # Paper: The packaged implementation applies a sigmoid to the paper's continuous-phenotype linear score, returning a bounded unitless score.\n",
    "model.metadata[\"model_type\"] = \"LASSO regression\"  # Paper: The glmnet mixing parameter alpha was set to 1, applying a LASSO penalty with tenfold cross-validation.\n",
    "model.metadata[\"platform\"] = [\"Illumina EPIC\"]  # Paper: Predictor training used quality-controlled HumanMethylationEPIC blood data; probes absent from 450K were filtered only to enable LBC1936 prediction.\n",
    "model.metadata[\"population\"] = \"adults\"  # Paper: The predictors were built on a subset of 5,087 Generation Scotland participants; the parent cohort spans ages 18–99.\n",
    "model.metadata[\"journal\"] = \"Genome Biology\"\n",
    "model.metadata[\"last_author\"] = \"Riccardo E. Marioni\"\n",
    "model.metadata[\"n_features\"] = 968\n",
    "model.metadata[\"citations\"] = 301\n",
    "model.metadata[\"citations_date\"] = \"2026-07-05\"\n"
   ]
  },
  {
   "cell_type": "markdown",
   "id": "74492239-5aae-4026-9d90-6bc9c574c110",
   "metadata": {},
   "source": [
    "## Download clock dependencies"
   ]
  },
  {
   "cell_type": "markdown",
   "id": "7bec474f-80ce-4884-9472-30c193327117",
   "metadata": {},
   "source": [
    "#### Download coefficient file"
   ]
  },
  {
   "cell_type": "code",
   "execution_count": 9,
   "id": "aa4a1b59-dda3-4ea8-8f34-b3c53ecbc310",
   "metadata": {
    "execution": {
     "iopub.execute_input": "2025-04-07T17:47:35.492080Z",
     "iopub.status.busy": "2025-04-07T17:47:35.491992Z",
     "iopub.status.idle": "2025-04-07T17:47:36.204837Z",
     "shell.execute_reply": "2025-04-07T17:47:36.204380Z"
    }
   },
   "outputs": [
    {
     "data": {
      "text/plain": [
       "0"
      ]
     },
     "execution_count": 9,
     "metadata": {},
     "output_type": "execute_result"
    }
   ],
   "source": [
    "coeff_url = \"https://raw.githubusercontent.com/bio-learn/biolearn/master/biolearn/data/BodyFatMcCartney.csv\"\n",
    "os.system(f\"curl -L {coeff_url} -o BodyFatMcCartney.csv\")\n"
   ]
  },
  {
   "cell_type": "markdown",
   "id": "5035b180-3d1b-4432-8ebe-b9c92bd93a7f",
   "metadata": {},
   "source": [
    "## Load features"
   ]
  },
  {
   "cell_type": "markdown",
   "id": "15f4af76-b93c-438c-b57f-f129d6e9ec99",
   "metadata": {},
   "source": [
    "#### From CSV file"
   ]
  },
  {
   "cell_type": "code",
   "execution_count": 10,
   "id": "f26a49e3-7389-416c-9080-539f50e9abd0",
   "metadata": {
    "execution": {
     "iopub.execute_input": "2025-04-07T17:47:36.206985Z",
     "iopub.status.busy": "2025-04-07T17:47:36.206832Z",
     "iopub.status.idle": "2025-04-07T17:47:36.211174Z",
     "shell.execute_reply": "2025-04-07T17:47:36.210783Z"
    }
   },
   "outputs": [],
   "source": [
    "coeffs = pd.read_csv('BodyFatMcCartney.csv')\n",
    "coeffs['feature'] = coeffs.iloc[:,0]\n",
    "coeffs['coefficient'] = coeffs.iloc[:,1]\n",
    "\n",
    "model.features = coeffs['feature'].tolist()\n"
   ]
  },
  {
   "cell_type": "markdown",
   "id": "ee6d8fa0-4767-4c45-9717-eb1c95e2ddc0",
   "metadata": {},
   "source": [
    "## Load weights into base model"
   ]
  },
  {
   "cell_type": "markdown",
   "id": "d79e5690-e284-4de6-8460-d3545a8192af",
   "metadata": {},
   "source": [
    "#### From CSV file"
   ]
  },
  {
   "cell_type": "code",
   "execution_count": 11,
   "id": "7f6187ed-fcff-4ff2-bcb1-b5bcef8190e8",
   "metadata": {
    "execution": {
     "iopub.execute_input": "2025-04-07T17:47:36.213121Z",
     "iopub.status.busy": "2025-04-07T17:47:36.212960Z",
     "iopub.status.idle": "2025-04-07T17:47:36.216800Z",
     "shell.execute_reply": "2025-04-07T17:47:36.216396Z"
    }
   },
   "outputs": [],
   "source": [
    "weights = torch.tensor(coeffs['coefficient'].tolist()).unsqueeze(0)\n",
    "intercept = torch.tensor([0.0])\n"
   ]
  },
  {
   "cell_type": "markdown",
   "id": "ad261636-5b00-4979-bb1d-67a851f7aa19",
   "metadata": {},
   "source": [
    "#### Linear model"
   ]
  },
  {
   "cell_type": "code",
   "execution_count": 12,
   "id": "d7f43b99-26f2-4622-9a76-316712058877",
   "metadata": {
    "execution": {
     "iopub.execute_input": "2025-04-07T17:47:36.218646Z",
     "iopub.status.busy": "2025-04-07T17:47:36.218507Z",
     "iopub.status.idle": "2025-04-07T17:47:36.221310Z",
     "shell.execute_reply": "2025-04-07T17:47:36.220958Z"
    }
   },
   "outputs": [],
   "source": [
    "base_model = pya.models.LinearModel(input_dim=len(model.features))\n",
    "\n",
    "base_model.linear.weight.data = weights.float()\n",
    "base_model.linear.bias.data = intercept.float()\n",
    "\n",
    "model.base_model = base_model"
   ]
  },
  {
   "cell_type": "markdown",
   "id": "ad8b4c1d-9d57-48b7-9a30-bcfea7b747b1",
   "metadata": {},
   "source": [
    "## Load reference values"
   ]
  },
  {
   "cell_type": "code",
   "execution_count": 13,
   "id": "90d45266-962d-41b6-927c-6a147ed41305",
   "metadata": {
    "execution": {
     "iopub.execute_input": "2025-04-07T17:47:36.222862Z",
     "iopub.status.busy": "2025-04-07T17:47:36.222749Z",
     "iopub.status.idle": "2025-04-07T17:47:36.224649Z",
     "shell.execute_reply": "2025-04-07T17:47:36.224309Z"
    }
   },
   "outputs": [],
   "source": [
    "model.reference_values = None"
   ]
  },
  {
   "cell_type": "markdown",
   "id": "af3bcf7b-74a8-4d21-9ccb-4de0c2b0516b",
   "metadata": {},
   "source": [
    "## Load preprocess and postprocess objects"
   ]
  },
  {
   "cell_type": "code",
   "execution_count": 14,
   "id": "f7d32b69-e20e-42ff-aba9-d07b9b44dbd1",
   "metadata": {
    "execution": {
     "iopub.execute_input": "2025-04-07T17:47:36.226270Z",
     "iopub.status.busy": "2025-04-07T17:47:36.226156Z",
     "iopub.status.idle": "2025-04-07T17:47:36.228064Z",
     "shell.execute_reply": "2025-04-07T17:47:36.227750Z"
    }
   },
   "outputs": [],
   "source": [
    "model.preprocess_name = None\n",
    "model.preprocess_dependencies = None\n"
   ]
  },
  {
   "cell_type": "code",
   "execution_count": 15,
   "id": "7a22fb20-c605-424d-8efb-7620c2c0755c",
   "metadata": {
    "execution": {
     "iopub.execute_input": "2025-04-07T17:47:36.229604Z",
     "iopub.status.busy": "2025-04-07T17:47:36.229468Z",
     "iopub.status.idle": "2025-04-07T17:47:36.231233Z",
     "shell.execute_reply": "2025-04-07T17:47:36.230916Z"
    }
   },
   "outputs": [],
   "source": [
    "model.postprocess_name = \"sigmoid\"\n",
    "model.postprocess_dependencies = None\n"
   ]
  },
  {
   "cell_type": "markdown",
   "id": "86e3d6b1-e67e-4f3d-bd39-0ebec5726c3c",
   "metadata": {},
   "source": [
    "## Check all clock parameters"
   ]
  },
  {
   "cell_type": "code",
   "execution_count": 16,
   "id": "2168355c-47d9-475d-b816-49f65e74887c",
   "metadata": {
    "execution": {
     "iopub.execute_input": "2025-04-07T17:47:36.232808Z",
     "iopub.status.busy": "2025-04-07T17:47:36.232688Z",
     "iopub.status.idle": "2025-04-07T17:47:36.236913Z",
     "shell.execute_reply": "2025-04-07T17:47:36.236596Z"
    }
   },
   "outputs": [
    {
     "name": "stdout",
     "output_type": "stream",
     "text": [
      "\n",
      "%==================================== Model Details ====================================%\n",
      "Model Attributes:\n",
      "\n",
      "training: True\n",
      "metadata: {'approved_by_author': '⌛',\n",
      " 'citation': 'McCartney, Daniel L., et al. \"Epigenetic prediction of complex '\n",
      "             'traits and death.\" Genome biology 19.1 (2018): 136.',\n",
      " 'clock_name': 'mccartneybodyfat',\n",
      " 'data_type': 'methylation',\n",
      " 'doi': 'https://doi.org/10.1186/s13059-018-1514-1',\n",
      " 'notes': 'DNAm score for body fat percentage; logistic transform.',\n",
      " 'research_only': None,\n",
      " 'species': 'Homo sapiens',\n",
      " 'version': None,\n",
      " 'year': 2018}\n",
      "reference_values: None\n",
      "preprocess_name: None\n",
      "preprocess_dependencies: None\n",
      "postprocess_name: 'sigmoid'\n",
      "postprocess_dependencies: None\n",
      "features: ['cg06500161', 'cg05744675', 'cg06568506', 'cg06192883', 'cg25158622', 'cg21176130', 'cg04086445', 'cg26033520', 'cg05468843', 'cg20347986', 'cg17500119', 'cg00701514', 'cg09848445', 'cg11202345', 'cg20495962', 'cg03234777', 'cg17150306', 'cg18853935', 'cg19887178', 'cg17287155', 'cg01937601', 'cg17473923', 'cg20054939', 'cg00799631', 'cg10409248', 'cg26983874', 'cg10196309', 'cg25921813', 'cg27396655', 'cg08858160']... [Total elements: 968]\n",
      "base_model_features: None\n",
      "\n",
      "%==================================== Model Details ====================================%\n",
      "Model Structure:\n",
      "\n",
      "base_model: LinearModel(\n",
      "  (linear): Linear(in_features=968, out_features=1, bias=True)\n",
      ")\n",
      "\n",
      "%==================================== Model Details ====================================%\n",
      "Model Parameters and Weights:\n",
      "\n",
      "base_model.linear.weight: [17.009260177612305, 13.952584266662598, 13.8219633102417, 12.232629776000977, 12.198307037353516, 12.024375915527344, 10.987481117248535, 10.905646324157715, 10.629822731018066, 10.109126091003418, 9.78788948059082, 9.1156005859375, 8.941770553588867, 8.878946304321289, 8.463309288024902, 7.700728416442871, 7.354093551635742, 6.861233234405518, 6.658877849578857, 6.449416160583496, 6.411191940307617, 6.20827054977417, 6.0269551277160645, 5.763856410980225, 5.442668914794922, 5.3892903327941895, 5.105152130126953, 4.995013236999512, 4.9773173332214355, 4.972265720367432]... [Tensor of shape torch.Size([1, 968])]\n",
      "base_model.linear.bias: tensor([0.])\n",
      "\n",
      "%==================================== Model Details ====================================%\n",
      "\n"
     ]
    }
   ],
   "source": [
    "pya.utils.print_model_details(model)"
   ]
  },
  {
   "cell_type": "markdown",
   "id": "9f71d32e",
   "metadata": {},
   "source": [
    "## Basic Test"
   ]
  },
  {
   "cell_type": "code",
   "execution_count": 17,
   "id": "936b9877-d076-4ced-99aa-e8d4c58c5caf",
   "metadata": {
    "execution": {
     "iopub.execute_input": "2025-04-07T17:47:36.238465Z",
     "iopub.status.busy": "2025-04-07T17:47:36.238354Z",
     "iopub.status.idle": "2025-04-07T17:47:36.242868Z",
     "shell.execute_reply": "2025-04-07T17:47:36.242565Z"
    }
   },
   "outputs": [
    {
     "data": {
      "text/plain": [
       "tensor([[4.7729e-13],\n",
       "        [1.0000e+00],\n",
       "        [9.8856e-01],\n",
       "        [5.2320e-71],\n",
       "        [1.0000e+00],\n",
       "        [1.0000e+00],\n",
       "        [1.0431e-36],\n",
       "        [1.0000e+00],\n",
       "        [2.3326e-63],\n",
       "        [1.0000e+00]], dtype=torch.float64, grad_fn=<SigmoidBackward0>)"
      ]
     },
     "execution_count": 17,
     "metadata": {},
     "output_type": "execute_result"
    }
   ],
   "source": [
    "torch.manual_seed(42)\n",
    "input = torch.randn(10, len(model.features), dtype=float)\n",
    "model.eval()\n",
    "model.to(float)\n",
    "pred = model(input)\n",
    "pred"
   ]
  },
  {
   "cell_type": "markdown",
   "id": "fe8299d7-9285-4e22-82fd-b664434b4369",
   "metadata": {},
   "source": [
    "## Save torch model\n"
   ]
  },
  {
   "cell_type": "code",
   "execution_count": 18,
   "id": "5ef2fa8d-c80b-4fdd-8555-79c0d541788e",
   "metadata": {
    "execution": {
     "iopub.execute_input": "2025-04-07T17:47:36.244263Z",
     "iopub.status.busy": "2025-04-07T17:47:36.244159Z",
     "iopub.status.idle": "2025-04-07T17:47:36.246682Z",
     "shell.execute_reply": "2025-04-07T17:47:36.246395Z"
    }
   },
   "outputs": [],
   "source": [
    "torch.save(model, f\"../weights/{model.metadata['clock_name']}.pt\")"
   ]
  },
  {
   "cell_type": "markdown",
   "id": "bac6257b-8d08-4a90-8d0b-7f745dc11ac1",
   "metadata": {},
   "source": [
    "\n",
    "## Clear directory\n",
    "<a id=\"10\"></a>\n"
   ]
  },
  {
   "cell_type": "code",
   "execution_count": 19,
   "id": "11aeaa70-44c0-42f9-86d7-740e3849a7a6",
   "metadata": {
    "execution": {
     "iopub.execute_input": "2025-04-07T17:47:36.248184Z",
     "iopub.status.busy": "2025-04-07T17:47:36.248077Z",
     "iopub.status.idle": "2025-04-07T17:47:36.255709Z",
     "shell.execute_reply": "2025-04-07T17:47:36.255449Z"
    }
   },
   "outputs": [
    {
     "name": "stdout",
     "output_type": "stream",
     "text": [
      "Deleted file: BodyFatMcCartney.csv\n"
     ]
    }
   ],
   "source": [
    "# Function to remove a folder and all its contents\n",
    "def remove_folder(path):\n",
    "    try:\n",
    "        shutil.rmtree(path)\n",
    "        print(f\"Deleted folder: {path}\")\n",
    "    except Exception as e:\n",
    "        print(f\"Error deleting folder {path}: {e}\")\n",
    "\n",
    "# Get a list of all files and folders in the current directory\n",
    "all_items = os.listdir('.')\n",
    "\n",
    "# Loop through the items\n",
    "for item in all_items:\n",
    "    # Check if it's a file and does not end with .ipynb\n",
    "    if os.path.isfile(item) and not item.endswith('.ipynb'):\n",
    "        os.remove(item)\n",
    "        print(f\"Deleted file: {item}\")\n",
    "    # Check if it's a folder\n",
    "    elif os.path.isdir(item):\n",
    "        remove_folder(item)"
   ]
  }
 ],
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