Horvath2013

Index

1. Instantiate model class

2. Define clock metadata

3. Download clock dependencies

4. Load features

5. Load weights into base model

6. Load reference values

7. Load preprocess and postprocess objects

8. Check all clock parameters

9. Basic test

10. Save torch model

11. Clear directory

Let’s first import some packages:

import os
import inspect
import shutil
import json
import torch
import pandas as pd
import pyaging as pya

Instantiate model class

def print_entire_class(cls):
    source = inspect.getsource(cls)
    print(source)

print_entire_class(pya.models.Horvath2013)

class Horvath2013(pyagingModel):
    def __init__(self):
        super().__init__()

    def preprocess(self, x):
        return x

    def postprocess(self, x):
        """
        Applies an anti-logarithmic linear transformation to a PyTorch tensor.
        """
        adult_age = 20

        # Create a mask for negative and non-negative values
        mask_negative = x < 0
        mask_non_negative = ~mask_negative

        # Initialize the result tensor
        age_tensor = torch.empty_like(x)

        # Exponential transformation for negative values
        age_tensor[mask_negative] = (1 + adult_age) * torch.exp(x[mask_negative]) - 1

        # Linear transformation for non-negative values
        age_tensor[mask_non_negative] = (1 + adult_age) * x[mask_non_negative] + adult_age

        return age_tensor

model = pya.models.Horvath2013()

Define clock metadata

model.metadata["clock_name"] = 'horvath2013'
model.metadata["data_type"] = 'methylation'
model.metadata["species"] = 'Homo sapiens'
model.metadata["year"] = 2013
model.metadata["approved_by_author"] = '⌛'
model.metadata["citation"] = "Horvath, Steve. \"DNA methylation age of human tissues and cell types.\" Genome biology 14.10 (2013): 1-20."
model.metadata["doi"] = "https://doi.org/10.1186/gb-2013-14-10-r115"
model.metadata["research_only"] = None
model.metadata["notes"] = None

Download clock dependencies

Download directly with curl

supplementary_url = "https://static-content.springer.com/esm/art%3A10.1186%2Fgb-2013-14-10-r115/MediaObjects/13059_2013_3156_MOESM3_ESM.csv"
supplementary_file_name = "coefficients.csv"
os.system(f"curl -o {supplementary_file_name} {supplementary_url}")

0

supplementary_url = "https://static-content.springer.com/esm/art%3A10.1186%2Fgb-2013-14-10-r115/MediaObjects/13059_2013_3156_MOESM22_ESM.csv"
supplementary_file_name = "reference_feature_values.csv"
os.system(f"curl -o {supplementary_file_name} {supplementary_url}")

0

Load features

From CSV file

df = pd.read_csv('coefficients.csv', skiprows=2)
df['feature'] = df['CpGmarker']
df['coefficient'] = df['CoefficientTraining']
model.features = df['feature'][1:].tolist()

Load weights into base model

weights = torch.tensor(df['coefficient'][1:].tolist()).unsqueeze(0)
intercept = torch.tensor([df['coefficient'][0]])

Linear model

base_model = pya.models.LinearModel(input_dim=len(model.features))

base_model.linear.weight.data = weights.float()
base_model.linear.bias.data = intercept.float()

model.base_model = base_model

Load reference values

From CSV file

reference_feature_values_df = pd.read_csv('reference_feature_values.csv', index_col=0)
reference_feature_values_df = reference_feature_values_df.loc[model.features]
model.reference_values = reference_feature_values_df['goldstandard2'].tolist()

Load preprocess and postprocess objects

model.preprocess_name = None
model.preprocess_dependencies = None

model.postprocess_name = 'anti_log_linear'
model.postprocess_dependencies = None

Check all clock parameters

pya.utils.print_model_details(model)

%==================================== Model Details ====================================%
Model Attributes:

training: True
metadata: {'approved_by_author': '⌛',
 'citation': 'Horvath, Steve. "DNA methylation age of human tissues and cell '
             'types." Genome biology 14.10 (2013): 1-20.',
 'clock_name': 'horvath2013',
 'data_type': 'methylation',
 'doi': 'https://doi.org/10.1186/gb-2013-14-10-r115',
 'notes': None,
 'research_only': None,
 'species': 'Homo sapiens',
 'version': None,
 'year': 2013}
reference_values: [0.790221397, 0.89001929, 0.059106387, 0.23651937, 0.073668777, 0.563295909, 0.864999404, 0.027047887, 0.660721193, 0.033420176, 0.047913033, 0.517283973, 0.050756537, 0.072267723, 0.014877693, 0.876157036, 0.187082703, 0.170303406, 0.019217238, 0.560726569, 0.845964086, 0.447995921, 0.055406903, 0.059821557, 0.533869814, 0.065190933, 0.896227421, 0.090932158, 0.032431793, 0.480007151]... [Total elements: 353]
preprocess_name: None
preprocess_dependencies: None
postprocess_name: 'anti_log_linear'
postprocess_dependencies: None
features: ['cg00075967', 'cg00374717', 'cg00864867', 'cg00945507', 'cg01027739', 'cg01353448', 'cg01584473', 'cg01644850', 'cg01656216', 'cg01873645', 'cg01968178', 'cg02085507', 'cg02154074', 'cg02217159', 'cg02331561', 'cg02332492', 'cg02364642', 'cg02388150', 'cg02479575', 'cg02489552', 'cg02580606', 'cg02654291', 'cg02827112', 'cg02972551', 'cg03103192', 'cg03167275', 'cg03270204', 'cg03565323', 'cg03588357', 'cg03760483']... [Total elements: 353]
base_model_features: None

%==================================== Model Details ====================================%
Model Structure:

base_model: LinearModel(
  (linear): Linear(in_features=353, out_features=1, bias=True)
)

%==================================== Model Details ====================================%
Model Parameters and Weights:

base_model.linear.weight: [0.12933661043643951, 0.005017857067286968, 1.599764108657837, 0.056852418929338455, 0.10286285728216171, 0.23856045305728912, 0.08862839639186859, 0.1599487066268921, 0.04280552640557289, -0.6040563583374023, 1.1692458391189575, 0.006127551198005676, 0.04475700482726097, -0.08651260286569595, 0.12692885100841522, 0.06277134269475937, -0.024270255118608475, 0.42597126960754395, 1.8754462003707886, 0.0737413838505745, 0.34927448630332947, 0.14058348536491394, 0.05064608156681061, 0.4739460051059723, 0.02353634312748909, -0.5510412454605103, 0.013544725254178047, -0.2076532244682312, -0.8588430285453796, 0.014946399256587029]... [Tensor of shape torch.Size([1, 353])]
base_model.linear.bias: tensor([0.6955])

%==================================== Model Details ====================================%

Basic test

torch.manual_seed(42)
input = torch.randn(10, len(model.features), dtype=float)
model.eval()
model.to(float)
pred = model(input)
pred

tensor([[ -0.9718],
        [  1.8615],
        [ -0.9980],
        [ 67.1194],
        [253.6647],
        [ 61.3025],
        [ -0.9928],
        [ -0.6078],
        [ -0.9995],
        [ -0.7832]], dtype=torch.float64, grad_fn=<IndexPutBackward0>)

Save torch model

torch.save(model, f"../weights/{model.metadata['clock_name']}.pt")

Clear directory

# Function to remove a folder and all its contents
def remove_folder(path):
    try:
        shutil.rmtree(path)
        print(f"Deleted folder: {path}")
    except Exception as e:
        print(f"Error deleting folder {path}: {e}")

# Get a list of all files and folders in the current directory
all_items = os.listdir('.')

# Loop through the items
for item in all_items:
    # Check if it's a file and does not end with .ipynb
    if os.path.isfile(item) and not item.endswith('.ipynb'):
        os.remove(item)
        print(f"Deleted file: {item}")
    # Check if it's a folder
    elif os.path.isdir(item):
        remove_folder(item)

Deleted file: coefficients.csv
Deleted file: reference_feature_values.csv
Deleted file: coefficients.xlsx